ABCG1 Antibody (Center)

SKU: orb1930010

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB19679
• Target: This ABCG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-387 amino acids from the Central region of human ABCG1.
• Molecular Weight: 75592 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8, White protein homolog, ABCG1, ABC8, WHT1

ABCG1 Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ABCG1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-ABCG1 Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This ABCG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-387 amino acids from the Central region of human ABCG1.

UniProt

P45844

RefSeq (Protein):NP_997057.1, NP_997512.1, NP_997510.1, NP_058198.2, NP_997511.1, NP_004906.3