A-RAF Antibody (ARAF1) (Center)

SKU: orb1928868

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50~100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB03325
• Target: This A-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-286 amino acids from the Central region of human A-RAF.
• Molecular Weight: 67585 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Serine/threonine-protein kinase A-Raf, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2

Western blot analysis of hARAF1-H270 in MCF7 cell line lysates (35 ug/lane). ARAF1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

This A-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-286 amino acids from the Central region of human A-RAF.

UniProt

P10398

RefSeq (Protein):NP_001243125.1, NP_001645.1, NP_001243126.1