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A-RAF Antibody (ARAF1) (Center)
SKU: orb38545
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Description
Research Area
Cancer Biology, Immunology & Inflammation, Immunology & inflammation, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:50-100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB03325 |
| Immunogen | Synthetic Peptide |
| Target | ARAF |
| Molecular Weight | 67585 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-"A-Raf proto-oncogene serine/threonine-protein kinase antibody, anti-A-raf-1 antibody, anti-Proto-oncogene Pks" antibody
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Western blot analysis of hARAF1-H270 in MCF7 cell line lysates (35 ug/lane). ARAF1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001243125.1, NP_001645.1, NP_001243126.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
A-RAF Antibody (ARAF1) (Center) (orb38545)
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