5-Ethynyl-dUTP (5-EdUTP)

• Catalog Number: orb1734885
• Category: Tools
• Description: 5-Ethynyl-dUTP (5-EdUTP)
• Concentration: 100 mM-110 mM
• Form/Appearance: solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 492.16 g/mol (free acid); Detected MW: 491.97 g/mol (free acid)
• SMILES: c1(=O)[nH]c(=O)n(cc1C#C)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O
• Formula: C₁₁H₁₅N₂O₁₄P₃
• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Note: For research use only
• Application notes: Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Ventexo-or KOD XL). Incorporation into DNA by Primer Extension with family A polymerase Taqand family B polymerases (Pwo, Deep Vent exo-or KOD XL). The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins. < br>< b>Spectroscopic Propertie: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• Expiration Date: 12 months from date of receipt.