5-Ethynyl-dUTP (5-EdUTP)

SKU: orb532423

Description

5-Ethynyl-dUTP (5-EdUTP)

Images & Validation

• Application Notes:
  Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Ventexo-or KOD XL). Incorporation into DNA by Primer Extension with family A polymerase Taqand family B polymerases (Pwo, Deep Vent exo-or KOD XL). The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins. Spectroscopic Propertie: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 492.16 g/mol (free acid); Detected MW: 491.97 g/mol (free acid)
• Purity: 95% (HPLC)
• Formula: C₁₁H₁₅N₂O₁₄P₃
• SMILES: c1(=O)[nH]c(=O)n(cc1C#C)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O

Storage & Handling

• Storage: store at -20 C
• Disclaimer: For research use only