5-Chloro-2-deoxyuridine

SKU: orb1940620

Description

5-Chloro-2'-deoxyuridine is a thymine analog. It can be used to study the potential of hypochlorous acid damage to DNA and DNA precursors.(In Vitro):When 5-Chloro-2’-deoxyuridine (ClDU) is placed into the tissue culture medium, mammalian cells incorporate the analog into DNA. It is observed that a 10 μM concentration of 5-Chloro-2’-deoxyuridine in the media does not alter cell division kinetics. Previously it has been shown that 5-Chloro-2’-deoxyuridine is metabolized and incorporated into DNA using antibodies that bind selectively to DNA containing halogenated bases. In the studies reported here, 5-Chloro-2’-deoxyuridine is more similar to BrdU in acting as a T analog. The toxicity of 5-Chloro-2’-deoxyuridine could in part be attributed to inhibition of thymidylate synthase .

Key Properties

• CAS Number: 50-90-8
• MW: 262.65
• Purity: >98% (HPLC)
• Formula: C₉H₁₁ClN₂O₅
• SMILES: OC[C@H]1O[C@H](C[C@@H]1O)n1cc(Cl)c(=O)[nH]c1=O
• Target: TRPM4
• Solubility: In Vitro: DMSO : 250 mg/mL (951.84 mM)

Bioactivity

• In Vitro:
  When 5-Chloro-2’-deoxyuridine (ClDU) is placed into tissue culture medium, mammalian cells incorporate the analog into DNA. It is observed that 10 μM concentration of 5-Chloro-2’-deoxyuridine in the media does not alter cell division kinetics. Previously it has been shown that 5-Chloro-2’-deoxyuridine is metabolized and incorporated into DNA using antibodies that bind selectively to DNA containing halogenated bases. In the studies reported here, 5-Chloro-2’-deoxyuridine is more similar to BrdU in acting as a T analog. The toxicity of 5-Chloro-2’-deoxyuridine could in part be attributed to inhibition of thymidylate synthase.
  Read more...

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5-Chlorodeoxyuridine | CldU

Quick Database Links

Gene Symbol

TRPM4