2',5'-Dideoxyadenosine

SKU: orb1219956

Description

2',5'-Dideoxyadenosine is a potent and non-competitive adenylyl cyclase inhibitor via binding the P-site (IC50: 3 μM).2',5'-Dideoxyadenosine (20-150 mM), like adenosine, dependently and reversibly inhibits the positive inotropic and chronotropic effect of beta-adrenergic stimulation with isoproterenol (8-54 pmol) up to 70% and 50%, respectively [2]. 2',5'-Dideoxyadenosine (10 μM, 30 min) reduces cAMP production and blocks the phosphorylation of GluA1 at Ser845 induced by carbachol (CCh). 2',5'-Dideoxyadenosine (10 μM, 30 min) blocks CCh-induced increase of phosphorylation of Akt and attenuates CCh-induced phosphorylation of Ser2448 [3].2',5'-Dideoxyadenosine (0.1 mg/kg; i.p.; 15 min pre-treated) fully inhibits the natriuretic, diuretic and K+ and Cl- sparing effect of FrEtOAc in rats.

Key Properties

• CAS Number: 6698-26-6
• MW: 235.24
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₃N₅O₂
• SMILES: C[C@H]1O[C@H](C[C@@H]1O)n1cnc2c(N)ncnc12
• Target: Adenylyl cyclase (AC)
• Solubility: In Vitro: DMSO : 125 mg/mL (531.37 mM)

Bioactivity

• In Vivo:
  Animal model: Male Wistar rats (3-4 months old). Dosage: 0.1 mg/kg. Administration: IP; 15 min pre-treated. Result: Fully inhibited the diuretic, natriuretic and K+ and Cl-sparing effect of Fr EtOAc in rats.
• In Vitro:
  Western blot analysis. Cell line: Primary hippocampal neurons. Concentration: 10 μM. Incubation time: 30 min. Result: Reduced cAMP production and blocked the phosphorylation of GluA1 at Ser845 induced by carbachol (CCh).

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Adenylyl cyclase (AC)