14-3-3 ζ (A79) polyclonal antibody

SKU: orb643520

Description

Rabbit polyclonal antibody to 14-3-3 Zeta (A79)

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:500-1:1000, IHC-P: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 49-100 of Human 14-3-3 ζ
• Target: 14-3-3 ζ (A79) polyclonal antibody detects endogenous levels of 14-3-3 protein zeta/delta
• Molecular Weight: 28 kDa
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-14-3-3 protein zeta/delta antibody, anti-Protein kinase C inhibitor protein 1 antibody, anti-KCIP-1 antibody, anti-YWHAZ antibody, anti-14-3-3 protein zeta antibody, anti-14-3-3 protein delta antibody, anti-14-3-3 Zeta antibody, anti-14-3-3 Delta antibody, anti-1433Z antibody, anti-KCIP1 antibody, anti-KCIP 1 antibody

Western blot (WB) analysis of 14-3-3 ζ (A79) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:K562 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:3T3-L1 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (A79) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase

Quick Database Links

Gene Symbol

14-3-3 ζ (A79) polyclonal antibody detects endogenous levels of 14-3-3 protein zeta/delta

UniProt

P63104