1-(4-Chloro-3-(trifluoromethyl)phenyl)-3-(4-(4-cyanophenoxy)phenyl)urea

SKU: orb1940396

Description

SC-1 is a a derivative of the multiple tyrosine kinase inhibitor sorafenib. SC-1 potently inhibits the phosphorylation of STAT3 by blocking STAT3 phosphorylation and activation, and induces apoptosis in hepatocellular carcinoma cell lines.(In Vitro):SC-1(1-10 μM; 48 hours; breast cancer cells) treatment demonstrates dose-dependent suppression of cell viability in all tested breast cancer cells. SC-1(1-10 μM; 36 hours; breast cancer cells) treatment induces potent apoptotic activity in association with downregulation of p-STAT3 and its downstream proteins cyclin D1 and survivin in a dose-dependent manner in breast cancer cell lines.(In Vivo):SC-1 showes efficacious antitumor activity and p-STAT3 downregulation in MDA-MB-468 xenograft tumors in vivo.

Key Properties

• CAS Number: 1313019-65-6
• MW: 431.8
• Purity: >98% (HPLC)
• Formula: C₂₁H₁₃ClF₃N₃O₂
• SMILES: N#Cc(cc1)ccc1Oc(cc1)ccc1NC(Nc(cc1)cc(C(F)(F)F)c1Cl)=O
• Target: Apoptosis
• Solubility: In Vitro: DMSO : 250 mg/mL (578.97 mM)

Bioactivity

• In Vivo:
  STAT3-IN-7 (10 mg/kg; oral gavage; daily; for 28 days; female NCr athymic nude mice) treatment shows efficacious antitumor activity and p-STAT3 downregulation in MDA-MB-468 xenograft tumors. Animal model: Female NCr athymic nude mice (4-6 weeks of age) injected with breast cancer cells. Dosage: 10 mg/kg. Administration: Oral gavage; daily; for 28 days. Result: Showed efficacious antitumor activity and p-STAT3 downregulation in MDA-MB-468 xenograft tumors.
• In Vitro:
  STAT3-IN-7 (SC-1; 1-10 μM; 48 hours; breast cancer cells) treatment demonstrates dose-dependent suppression of cell viability in all tested breast cancer cells. STAT3-IN-7 (SC-1; 1-10 μM; 36 hours; breast cancer cells) treatment induces potent apoptotic activity. STAT3-IN-7 (SC-1; 1-10 μM; 36 hours; breast cancer cells) treatment shows downregulation of p-STAT3 and its downstream proteins cyclin D1 and survivin in a dose-dependent manner in breast cancer cell lines. Cell Viability Assay Cell line: HCC-1937, MDA-MB-231, MDA-MB-468, MDA-MB-453, SK-BR3 and MCF-7 cells. Concentration: 1 μM, 2 μM, 5 μM, 7.5 μM, 10 μM Incubation time: 48 hours. Result: Demonstrated dose-dependent suppression of cell viability in all tested breast cancer cells. Apoptosis Analysis Cell line: HCC-1937, MDA-MB-231, MDA-MB-468, MDA-MB-453, SK-BR3 and MCF-7 cells. Concentration: 1 μM, 2 μM, 5 μM, 7.5 μM, 10 μM Incubation time: 36 hours. Result: Induced potent apoptotic activity. Western blot analysis. Cell line: HCC-1937, MDA-MB-231, MDA-MB-468, MDA-MB-453, SK-BR3 and MCF-7 cells. Concentration: 1 μM, 2 μM, 5 μM, 7.5 μM, 10 μM Incubation time: 36 hours. Result: Showed downregulation of p-STAT3 and its downstream proteins cyclin D1 and survivin in a dose-dependent manner in breast cancer cell lines.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SC-1

Quick Database Links

Gene Symbol

Apoptosis