ZNF81 Antibody (N-term)

SKU: orb1934803

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB36052
• Target: This ZNF81 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-197 amino acids from the N-terminal region of human ZNF81.
• Molecular Weight: 75960 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Zinc finger protein 81, HFZ20, ZNF81

ZNF81 Antibody (N-term) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the ZNF81 antibody detected the ZNF81 protein (arrow).

Fluorescent confocal image of U251 cell stained with ZNF81 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF81 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZNF81 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly.

Quick Database Links

Gene Symbol

This ZNF81 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-197 amino acids from the N-terminal region of human ZNF81.

UniProt

P51508

RefSeq (Protein):NP_009068.2