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Catalog Number | orb1934457 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB36605 |
Tested applications | IF, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 62081 Da |
Target | This ZNF155 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480-509 amino acids from the C-terminal region of human ZNF155. |
UniProt ID | Q12901 |
NCBI | NP_003436.3, NP_001247415.1, NP_932355.2, NP_001247416.1, NP_001247417.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Zinc finger protein 155, ZNF155 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ZNF155 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the ZNF155 antibody detected the ZNF155 protein (arrow).
Fluorescent confocal image of Hela cell stained with ZNF155 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF155 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZNF155 immunoreactivity is localized to Nucleus significantly.