Zidovudine

SKU: orb1308679

Description

Zidovudine

Research Area

Molecular Biology

Key Properties

• CAS Number: 30516-87-1
• MW: 267.24
• Purity: >98%
• Formula: C₁₀H₁₃N₅O₄
• SMILES: O=C1N([C@H]2C[C@H](N=[N+]=[N-])[C@@H](CO)O2)C=C(C)C(=O)N1
• Target: Reverse transcriptase inhibitor
• Solubility: Soluble in DMSO (up to at least 40 mg/ml) or in Water (up to 25 mg/ml with warming)

Bioactivity

• In Vivo:
  Intravitreal injection of Nucleoside Reverse Transcriptase Inhibitors (NRTIs) Lamivudine (3TC), Zidovudine (AZT), or Abacavir (ABC) significantly mitigates laser-induced choroidal neovascularization (CNV) in wild-type mice when compared to the PBS vehicle treatment. Additionally, these NRTIs lead to a notable reduction in the mean VEGF-A levels within the RPE/choroid area, which typically reach their highest concentration on day 3 post-laser injury, in treated eyes relative to control eyes in wild-type mice. This effect, however, does not extend to P2rx7 knockout (P2rx7-/-) mice.
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• Cell Research:
  Zidovudine is prepared in DMSO (10 mM) and stored, and then diluted with appropriate medium before use. Assays are performed in all cell types in the presence of titrating concentrations of ARV. 5,000 SVG, 2,500 PFA, 200,000 PBMC, or 50,000 MDM cells/well are seeded into triplicate wells of 96-well plates. Twenty-four hours later, the culture medium is removed and replaced with medium containing the ARV or DMSO (0.5% vol/vol), and equivalent TCID50 infectious units of luciferase reporter virus are added to the cells. After a 16 h incubation at 37°C, the initial viral inoculum is removed and replaced with culture medium containing the same antiretroviral drug (ARV) or DMSO (0.5% vol/vol) concentrations. At 72 h post infection, the medium is aspirated, the cells are lysed and HIV-1 infection measured using the Luciferase Assay System. Luminescence is measured using a FLUOStar Optima microplate reader. Inhibition curves and the 50% (EC50) and 90% (EC90) effective concentrations are determined by nonlinear regression analysis, using GraphPad Prism software.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Azidothymidine; AZT

Compound Identifiers

PubChem CID

35370