ZD7288

SKU: orb1299749

Description

ZD7288

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 133059-99-1
• MW: 292.81
• Purity: 99.99%
• Formula: C₁₅H₂₁ClN₄
• SMILES: Cl.CCN(c1ccccc1)c1cc(=NC)n(C)c(C)n1
• Target: HCN Channel
• Solubility: DMSO:225 mg/mL (768.42 mM);H2O:50 mg/mL (170.76 mM)

Bioactivity

• In Vitro:
  ZD7288 attenuated glutamate-induced rises in [Ca(2+)]i in a concentration-dependent manner and reversed 8-Br-cAMP-mediated facilitation of these glutamate-induced [Ca(2+)]i rises. ZD7288 inhibits hippocampal synaptic plasticity both glutamate release and resultant [Ca(2+)]i increases in rat hippocampal neurons.
• Cell Research:
  Cultured hippocampal neurons were incubated with 1 μM Fura-2 acetoxy-methylol ester for 30 minutes at 37°C, washed three times with artificial cerebrospinal fluid (containing 140 mM NaCl, 5 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 10 mM glucose and 10 mM hydroxyethyl piperazine ethanesulfonic acid, pH 7.3), and then incubated at room temperature in the dark for 30 minutes. Fura-2 fluorescence was observed by a Ratio Vision digital fluorescence microscopy system. Fluorescence signals were evoked by 340 and 380 nm excitation wavelengths and collected at 510 nm by TILLvisION 4.0 software. The 340:380 nm fluorescence ratio was used to represent [Ca2+]i. Peak calcium change was represented as the percentage increase from baseline. Neurons were incubated in ZD7288 (25, 50 or 100 μM) or 8-Br-cAMP (5 or 50 μM) for 15 minutes prior to stimulation with 50 μM glutamate. All experiments were repeated in triplicate, using different batches of cells across 4–5 dishes.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ZD 7288, ZD7288, ZD-7288, inhibit, Inhibitor, HCNChannel, HCN Channel, Hyperpolarization activated cyclic nucleotide gated channels, ICI D 7288, ICI D7288, ICI D-7288

Compound Identifiers

PubChem CID

123983