Z-DEVD-FMK

SKU: orb1301196

Description

Z-DEVD-FMK is a potent, cell-permeable, and irreversible inhibitor of Caspase-3 with an IC50 of 18 μM. It is widely used in apoptosis research to block caspase-dependent cell death in both cellular (in vitro) and animal (in vivo) experimental models.

Research Area

Cell Biology, Neuroscience

Key Properties

• CAS Number: 210344-95-9
• MW: 668.7
• Purity: >98%
• Formula: C₃₀H₄₁FN₄O₁₂
• SMILES: COC(=O)CC[C@H](NC(=O)[C@H](CC(=O)OC)NC(=O)OCc1ccccc1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(=O)OC)C(=O)CF
• Target: Protease
• Solubility: Soluble in DMSO (up to 20 mg/ml)

Bioactivity

• Target IC50:
  Caspase-3:18 μM.
• In Vivo:
  METHODS: To test the in vivo activity, Z-DEVD-FMK (1.8 mg/kg in ethanol and freshly diluted in PBS containing 2% Tween-80) was injected intraperitoneally into C57BL/6 mice, followed by CPT-11 (350 mg/kg) 30 min later. Z-DEVD-FMK was continued to be administered once daily for three days. RESULTS: Caspase-3 inhibitor Z-DEVD-FMK attenuated PT-11-induced GATA6-deficient mouse peritoneal macrophages.
• In Vitro:
  METHODS: v-K-ras transformed normal rat kidney cells KNRK were treated with SCH56582 (20 µM) and Z-DEVD-FMK (20-50 µM) for 24 h. Cell death was detected using trypan blue. RESULTS: Addition of 50 µM Z-DEVD-fmk resulted in >70% inhibition of apoptosis. METHODS: Human multiple myeloma cells KM3 were treated with betulinic acid (15 µg/mL) and Z-DEVD-FMK (50 mol/L) for 24 h, and the expression level of target proteins was detected using Western Blot. RESULTS: Z-DEVD-FMK attenuated the betulinic acid-induced activation of caspase 3. Z-DEVD-FMK significantly blocked the cleavage of PARP. Z-DEVD-FMK significantly blocked PARP cleavage.
• Cell Research:
  N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Caspase, Caspase-3, Caspase-3 Inhibitor, Inhibitor, inhibit, ZDEVDFMK, Z-DEVD-FMK, Z DEVD FMK

Compound Identifiers

PubChem CID

16760394