YY1 Antibody

• Catalog Number: orb1927299
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1183CT6.5.23.6
• Tested applications: IHC-P, WB
• Reactivity: Human, Monkey, Rat, Zebrafish
• Isotype: IgG2a
• Dilution range: WB: 1:500-1:1000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 44713 Da
• Target: Purified His-tagged YY1 protein was used to produced this monoclonal antibody.
• UniProt ID: P25490
• NCBI: NP_003394.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcriptional repressor protein YY1, Delta trans
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

YY1 Antibody western blot analysis in Daudi and U251 cell line lysates (35μg/lane).This demonstrates the YY1 antibody detected the YY1 protein (arrow).

All lanes: Anti-YY1 Antibody (Center) at 1:500-1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: C6 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: U-251 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining YY1 in Rat brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining YY1 in Monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining YY1 in Zebra fish brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.