YY1 Antibody / Ying-Yang 1

SKU: orb1825484

Description

The YY1 transcription factor, also known as NF-E1 (human) and Delta or UCRBP (mouse) is of interest due to its diverse effects on a wide variety of target genes. YY1 is broadly expressed in a wide range of cell types and contains four C-terminal zinc finger motifs of the Cys-Cys-His-His type and an unusual set of structural motifs at its N-terminal end. It binds to downstream elements in several vertebrate ribosomal protein genes, where it apparently acts positively to stimulate transcription and can act either negatively or positively in the context of the immunoglobulin k 3' enhancer and immunoglobulin heavy-chain �E1 site as well as the P5 promoter of the adenoassociated virus. It thus appears that YY1 is a bifunctional protein, capable of functioning as an activator in some transcriptional control elements and a repressor in others.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the YY1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2, kappa
• Clone No.: YY1/5436
• Immunogen: A His-tagged YY1 protein was used as the immunogen for the YY1 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human Daudi cell lysate with YY1 antibody (YY1/5436). Predicted molecular weight ~45 kDa but commonly observed at 45~65 kDa.

IHC staining of FFPE human tonsil tissue with YY1 antibody (YY1/5436). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free YY1 antibody (clone YY1/5436) as confirmation of integrity and purity.

Quick Database Links

UniProt

P25490