Yeast Poly(A) Polymerase

SKU: orb611233

Description

Yeast Poly(A) Polymerase catalyzes the transfer of AMP to 3'-hydroxyl ends of RNA molecules. The reaction is template-independent, requires ATP as substrate and Mg2+or Mn2+as cofactor. It works more efficiently than E.coli Poly(A) polymerase in some poly(A) tailing and RNA labeling reactions (e.g. shorter incubation time, broader acceptance of RNA template size). Polyadenylation increases (m)RNA stability and therefore translation efficiency in transfection and microinjection experiments in eukary-otic cells. For information on (m)RNA polyadenylation using in vitro tran-scription reaction mixes as template, refer to the Poly(A) Tailing Enzyme Testkit (#orb611231).

Research Area

Infectious Disease & Virology; Molecular Biology

Key Properties

• Purity: ≥ 95% (SDS-PAGE)

Storage & Handling

• Storage: store at -20 °C
• Form/Appearance: liquid
• Concentration: 600 units/µl
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

recombinant, E. coli overexpressing Saccharomyces cerevisiae Poly(A) Polymerase