XRCC5 Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1172974
• Category: Antibodies
• Description: XRCC5 Recombinant Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: F1D10
• Tested applications: ICC, WB
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG1/Kappa
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:200-1:1000, ICC/IF=1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: XRCC5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Lysate: HeLa, Jurkat, A549, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 86 kDa, Observed MW: 86 kDa.

Cell line: HeLa, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172974.