WDR70 Antibody (C-term)

• Catalog Number: orb1938249
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This WDR70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-604 amino acids from the C-terminal region of human WDR70.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9NW82
• MW: 73201 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: WB: 1:1000, IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB29043
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: WD repeat-containing protein 70, WDR70
• Research Area: Cell Biology
• Note: For research use only
• NCBI: NP_060504.1

Anti-WDR70 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 70 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of WDR70 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

WDR70 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

WDR70 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of WDR70 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

WDR70 Antibody (C-term) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the WDR70 antibody detected the WDR70 protein (arrow).