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Catalog Number | orb1260356 |
---|---|
Category | Antibodies |
Description | VTN Antibody |
Target | VTN |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 399-478 of human VTN (NP_000629.3). |
UniProt ID | P04004 |
MW | Observed: 70kDa |
Tested applications | IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Vitronectin, VN, S-protein, Serum-spreading factor Read more... |
Note | For research use only |
Western blot analysis of extracts of human serum, using VTN antibody (orb1260356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human mammary cancer using VTN antibody (orb1260356) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using VTN antibody (orb1260356) at dilution of 1:100 (40x lens).
IF, IHC-Fr, IHC-P | |
Bovine, Canine, Human, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |