VTN Antibody (C-term)

• Catalog Number: orb1929232
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This VTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-379 amino acids from the C-terminal region of human VTN.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P04004
• MW: 54306 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB17982
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Vitronectin, VN, S-protein, Serum-spreading factor
• Research Area: Cardiovascular Research, Immunology & Inflammation
• Note: For research use only
• NCBI: NP_000629.3
• Expiration Date: 12 months from date of receipt.

Western blot analysis of VTN Antibody (C-term) in NCI-H460 (lane 1), HepG2 (lane 2) cell line lysates (35 ug/lane). VTN (arrow) was detected using the purified Pab.

Flow cytometric analysis of NCI-H292 cells using VTN Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with VTN Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.