VlsE Antibody

SKU: orb344689

Description

VlsE antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:250, WB: 1:1000
• Reactivity: Bacteria
• Application Notes:
  Anti-VlsE antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~36.3 kDa in size corresponding to VlsE by Western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MBP recombinant protein corresponding to Borrelia burgdorferi VlsE protein.
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi VlsE protein. A BLAST analysis was used to suggest reactivity with VlsE from B. burgdorferi sources based on 100% homology with the immunizing sequence. Cross-reactivity with VlsE from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-VlsE Antibody, Outer surface protein VlsE, Borrelia burgdorferi VlsE, vlsE protein

Western blot showing detection of 0.1 µg of recombinant VlsE protein. Lane 1: Molecular weight markers. Lane 2: MBP-VlsE fusion protein (arrow; expected MW: 78.8 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n orb348540, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n orb347654) secondary antibody was used at 1:40000 in orb348637 blocking buffer.

Quick Database Links

UniProt

G5IXI6

RefSeq (Protein):WP_010890281.1