Vincristine sulfate

SKU: orb1309169

Description

Vincristine sulfate is a microtubule-destabilizing alkaloid that inhibits mitosis by binding tubulin. It is widely used in research models of hematologic cancers like leukemia and lymphoma, as well as in pediatric sarcoma studies, employing both in vitro and in vivo experimental approaches.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 2068-78-2
• MW: 923.04
• Purity: 99.94%
• Formula: C₄₆H₅₈N₄O₁₄S
• SMILES: OS(O)(=O)=O.CC[C@]1(O)C[C@H]2C[N@@](C1)CCc1c([nH]c3ccccc13)[C@@](C2)(C(=O)OC)c1cc2c(cc1OC)N(C=O)[C@@H]1[C@]22CCN3CC=C[C@](CC)([C@@H]23)[C@@H](OC(C)=O)[C@]1(O)C(=O)OC
• Target: Apoptosis,Microtubule Associated
• Solubility: H2O:92.3 mg/mL (100 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (2.17 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:50 mg/mL (54.17 mM)

Bioactivity

• Target IC50:
  Microtubule:85 nM (Ki, cell free)
• In Vivo:
  METHODS: To assay antitumor activity in vivo, Vincristine sulfate (0.025 mg/kg, intravenously, once weekly) and SAHA (200 mg/kg, orally, once daily) were administered to SCID mice bearing MOLT-4 xenografts for 24 days. RESULTS: TGD did not improve in mice treated with Vincristine or SAHA alone. However, log-rank analysis showed that co-treatment exhibited significant anti-tumor activity in the MOLT-4 xenograft model.
• In Vitro:
  METHODS: Neuroblastoma cells SH-SY5Y were treated with Vincristine sulfate (0.001-10 µM) for 24-72 h. Cell viability was measured by MTT assay. RESULTS: Vincristine inhibited the proliferation of SH-SY5Y cells in a dose- and time-dependent manner, with IC50s of 0.113 µM, 0.078 µM, and 0.051 µM at 24, 48, and 72 h, respectively. METHODS: Human leukemia cells MOLT-4 were treated with Vincristine sulfate (0.3-3 µM) and SAHA (500 nM) for 24-48 h. Cell cycle was detected using Flow cytometry. RESULTS: Vincristine treatment induced an increase in the G2/M phase of the cell cycle compared to SAHA. the combination of Vincristine plus SAHA resulted in almost complete cell arrest in the G2/M phase after short-term treatment (24 h), followed by induction of the cells into the sub-G1 phase after long-term treatment (48 h). the combination of Vincristine and SAHA resulted in an increase in the G2/M phase of the cell cycle compared to SAHA.
• Cell Research:
  SH-SY5Y cells at a logarithmic phase were seeded in 96-well plates (at 2x10^6/l) and incubated for 12 h until cells formed a monolayer. Wells were randomly chosen for treatment groups and a control group. For the treatment groups, cells were incubated with 200 μl of cell culture medium containing 0.001, 0.01, 0.1, 1 or 10 μM of VCR. In the control group, cells were grown in 200 μl cell culture medium only. Cells were incubated for another 24, 48 and 72 h and then 20 μl of 5 g/l MTT (0.1 mg/l final concentration) was added to each well. After 4 h of incubation, the cell culture supernatant was removed, 150 μl of DMSO was added to each well and the plate was shaken for 10 min. The absorbance of each well was detected at 490 nm (A value) on an ELISA plate reader. The growth inhibition rate of VCR-treated cells was calculated as: Growth inhibition rate % = [(average A value of control group - average A value of VCR-treated group)/average A value of control group] x 100%. This experiment was performed in triplicates .
• Animal Research:
  Vincristine (1 mg/ml) was diluted in saline and administered i.v. to wild-type and Mdr1ab/Mrp1 TKO mice, aged 10–14 weeks, at dose levels ranging between 0.125 and 4 mg/kg. Animals were monitored daily and killed when they lost more than 20% of their initial body weight. The MTD was defined as one dose step below the dose where more than one animal in that group had to be killed. Necropsies were performed in wild-type and Mdr1ab/Mrp1 TKO mice receiving vincristine at or near the MTD and killed 2 days later .

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, 22-Oxovincaleukoblastine, 22-Oxovincaleukoblastine sulfate, 22-Oxovincaleukoblastine Sulfate, Leurocristine, Leurocristine Sulfate, Leurocristine sulfate, NSC-67574, NSC67574, MicrotubuleAssociated, Microtubule Associated, Microtubule/Tubulin, microtubule, NSC 67574, Inhibitor, inhibit, Vincristine, Vincristine sulfate, Vincristine Sulfate

Compound Identifiers

PubChem CID

249332