Uridine

SKU: orb1306071

Description

Uridine is a ribonucleoside fundamental to RNA structure, where it base-pairs with adenine. This compound is widely used in research to study nucleotide metabolism, RNA synthesis, and cellular processes in both in vitro biochemical assays and in vivo metabolic studies.

Research Area

Cell Biology, Metabolism Research, Molecular Biology, Signal Transduction

Key Properties

• CAS Number: 58-96-8
• MW: 244.2
• Purity: 99.54% (May vary between batches)
• Formula: C₉H₁₂N₂O₆
• SMILES: OCC1O[C@H](C(O)[C@H]1O)N1C=CC(=O)NC1=O
• Target: Endogenous Metabolite
• Solubility: H2O:45.00 mg/mL (184.28 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5.00 mg/mL (20.48 mM);DMSO:240.00 mg/mL (982.80 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  RAW 264.7 cells:> 10 μM (EC50)|H9 cells:10 μg/mL|P2Y6:300 nM|P2Y14 receptor:7.28 (pEC₅₀)
• In Vivo:
  METHODS: To detect anti-tumor activity in vivo, Uridine (100-300 mg/kg) was injected locally into BALB/c nude mice bearing 97H xenografts once daily for sixteen days. RESULTS: Both doses of Uridine significantly reduced the tumor size of subcutaneous tumors in HCC nude mice in a dose-dependent manner.
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• In Vitro:
  METHODS: HCC cells HEPG2, HLF and 97H were treated with Uridine (0-600 µM) for 48 h. Cell viability was measured by CCK8 assay. RESULTS: The proliferative capacity of the three HCC cells was significantly inhibited in a dose-dependent manner. METHODS: Differentiated PC-12 cells were treated with Uridine (50 µM) for 4 days, and the expression levels of target proteins were measured by Western Blot. RESULTS: Uridine treatment enhanced neurofilament expression in differentiated PC12 cells, and NF-M and NF-70 expression were significantly increased after Uridine treatment.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

beta-Uridine, b-Uridine, NSC20256, NSC-20256, LSm6, NSC 20256, EndogenousMetabolite, Endogenous Metabolite, Uridin, β-Uridine

Compound Identifiers

PubChem CID

119106346