UHRF1 (vPairTM) Antibodies

SKU: orb131739

Description

Rabbit polyclonal to UHRF1. UHRF1 antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Biorbyt to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody pairs-based assays. The use of a pair of fully characterized vPair antibodies in the same assay can validate signal specificity since vPair antibodies recognize two independent epitopes of the same protein. Different sets of vPair antibodies are developed at Biorbyt to work with specific applications, including antibody pairs arrays, Western blot, IP-Western, ChIP, IHC, and FACS. E3 ubiquitin-protein ligase UHRF1 is a multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. UHRF1 specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, UHRF1 also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, UHRF1 specifically recognizes and binds histone H3 trimethylated at Lys-9 (H3K9me3) and unmethylated at Arg-2 (H3R2me0), respectively, and recruits chromatin proteins.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: WB
• Dilution Range: WB:1:1000-1:3000
• Reactivity: Human
• Application Notes:
  WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; ; Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the middle region or the C-terminal region of human UHRF1.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: middle region or the C-terminal region of human UHRF1
• Molecular Weight: 90
• Purity: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01% NaN3
• Concentration: 100 μg/ml BSA
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti E3 ubiquitin-protein ligase UHRF1 antibody pairs, anti Inverted CCAAT box-binding protein of 90 kDa antibody pairs, anti Nuclear protein 95 antibody pairs, anti Nuclear zinc finger protein Np95 antibody pairs, anti HuNp95 antibody pairs, anti hNp95 antibody pairs, anti RING finger protein 106 antibody pairs, anti Transcription factor ICBP90 antibody pairs, anti Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody pairs, anti hUHRF1 antibody pairs, anti Ubiquitin-likecontaining PHD and RING finger domains protein 1 antibody pairs, anti ICBP90 antibody pairs, anti NP95 antibody pairs, anti RNF106 antibody pairs

Quick Database Links

UniProt

Q96T88