UHRF1 (C) Antibody, Rabbit Polyclonal

SKU: orb131740

Description

Rabbit polyclonal to UHRF1. E3 ubiquitin-protein ligase UHRF1 is a multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. UHRF1 specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, UHRF1 also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, UHRF1 specifically recognizes and binds histone H3 trimethylated at Lys-9 (H3K9me3) and unmethylated at Arg-2 (H3R2me0), respectively, and recruits chromatin proteins.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: WB
• Dilution Range: WB:1:1000-1:3000
• Reactivity: Human
• Application Notes:
  WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; ; Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the middle region of human UHRF1.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: middle region of human UHRF1
• Molecular Weight: 90
• Purity: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01% NaN3
• Concentration: 100 μg/ml BSA
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti E3 ubiquitin-protein ligase UHRF1 antibody, anti Inverted CCAAT box-binding protein of 90 kDa antibody, anti Nuclear protein 95 antibody, anti Nuclear zinc finger protein Np95 antibody, anti HuNp95 antibody, anti hNp95 antibody, anti RING finger protein 106 antibody, anti Transcription factor ICBP90 antibody, anti Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody, anti hUHRF1 antibody, anti Ubiquitin-likecontaining PHD and RING finger domains protein 1 antibody, anti ICBP90 antibody, anti NP95 antibody, anti RNF106 antibody

Quick Database Links

UniProt

Q96T88