UDP-6-azide-glucose

• Catalog Number: orb532326
• Category: Tools
• Description: UDP-6-azide-glucose
• Form/Appearance: solid; Color: white to off-white
• Purity: ≥ 98% (HPLC)
• MW: Theoretical MW: 591.32 g/mol (free acid); Detected MW: 591.06 g/mol (free acid)
• SMILES: [C@H]1([C@H](C(O[C@@H]([C@H]1O)CN=[N+]=[N-])OP(=O)(OP(=O)(OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)n1c(=O)[nH]c(=O)cc1)O)O)O)O
• Formula: C₁₅H₂₃N₅O₁₆P₂
• Hazard Information: Non-Toxic
• Solubility (25°C): water, aqueous buffer such as PBS
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Glycosylation of 5-hydroxymethylcytosine (5-hmC) with T4 ß-Glycosyltransferase (T4 ß-GT). The resulting azide-functionalized, glycosylated 5-hmC moiety can subsequently be detected via Cu(I)-free click chemistry that offers the choice: to introduce a (Desthio)Biotin group for subsequent purification tasks (via DBCO-containing (Desthio) Biotin); to introduce fluorescent group for subsequent microscopic imaging (via DBCO-containing fluorescent dyes). 0.5 mg UDP-6-azide-glucose are sufficient to prepare 283 µl of a 3 mM solution.
• Expiration Date: 12 months from date of receipt.