UCHL1/PGP9.5 Antibody

• Catalog Number: orb1537022
• Category: Antibodies
• Description: UCHL1/PGP9.5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Bovine, Canine, Equine, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat
• Immunogen: Peptide with sequence C-QHENFRKKQIE, from the internal region of the protein sequence according to NP_004172.2.
• Concentration: 0.5 mg/ml
• Dilution range: ELISA, IHC-P (2.5 µg/ml), Peptide-ELISA (1:16000), WB (0.01 - 0.03 µg/ml)
• Conjugation: Unconjugated
• Target: UCHL1 / PGP9.5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: UCHL1, Ubiquitin C-terminal hydrolase, PARK5, PGP9
• Note: For research use only
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 26kD band observed in Human Brain (Cerebellum and Hippocampus) and Rat Brain lysates (calculated MW of 24.8kD according to NP_004172.2). Recommended concentration: 1-3 ug/ml.
• Expiration Date: 12 months from date of receipt.

Human Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

Goat Anti-UCHL1 (aa 58-68) Antibody (0.01 µg/ml) staining of Mouse (A) and Rat (B) Brain lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.