TPH2 Antibody

SKU: orb1246825

Description

TPH2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments in human brain lysates gave no specific signal but low background (at antibody concentration up to 1µg/mL).Immunohistochemistry:In paraffin embedded Human Brain Stem shows layered neuronal staining. Recommended concentration: 4-6µg/mL.Immunofluorescence:This product has been successfully used in IF on Mouse: Rahim RS et al, Mol Cell Neurosci. 2018 Apr;88:16-32. PMID: 29187321, and Rahim RS et al, Neuroscience 334 (2016) 201-213. PMID: 27514574.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NKPNSGKNDDKGNK
• Target: TPH2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TPH2, tryptophan hydroxylase 2, HGNC:20692, FLJ37295, NTPH , neuronal tryptophan hydroxylase, ADHD7, MGC138871, MGC138872, tryptophan 5-monooxygenase 2

orb1246825 (4 ug/ml) staining of paraffin embedded Human Brain Stem. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Quick Database Links

Gene Symbol

TPH2

UniProt

Q8IWU9

RefSeq (Protein):NP_775489.2