Toal Glutathione (T-GSH) Colorimetric Assay Kit

SKU: orb1173217

Description

GSSG is reduced to GSH using Glutathione Reductase (GR) for the determination of total glutathione. GSH reacts with 5,5'-dithiobis-bis-(2-nitrobenzoic acid) to form 2-nitrobenzoic acid, which has an absorption peak at 412 nm.

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Application Notes:
  GSSG is reduced to GSH using Glutathione Reductase (GR). GSH reacts with 5,5'-dithiobis-bis-(2-nitrobenoicacid) to form 2-nitrobenoicacid which has a characteristic absorption peak at 412 nm, the content of total glutathione is determined then. It can test samples of tissues, bacteria, fungus, cells, serums, ect.

Key Properties

• Sample Types: Animal and Plant Tissues, Bacteria, Cells and Serum
• Range: 0.04-4ug/mL
• Sensitivity: 0.04ug/mL

Storage & Handling

• Storage: Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
• Expiration Date: 6 months from date of receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only

Alternative Names

Toal Glutathione; T-GSH; t-GSH