TLR7 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1974655
• Category: Antibodies
• Description: TLR7 Recombinant Rabbit Monoclonal Antibody
• Target: TLR7
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human TLR7 protein (24-348/1049aa)
• UniProt ID: Q9NYK1
• MW: 121 kDa
• Tested applications: FC, WB
• Dilution range: Flow-Cyt=1ug/Test, WB=1:500-2000
• Antibody Type: Recombinant Antibody
• Clone Number: 8H7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TLR-7; PRO285; TLR 7; TLR7_HUMAN; Toll like recept
• Note: For research use only

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-TLR7 antibody (orb1974655), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Recombinant human TLR7 protein, His, Primary: Anti-TLR7 (orb1974655) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kDa, Observed band size: 40 kDa.