TLR7 Recombinant Rabbit Monoclonal Antibody

SKU: orb1974655

Description

TLR7 Recombinant Rabbit Monoclonal Antibody

Research Area

Developmental Biology, Inflammation, Macrophage, Signal Transduction, TLR Signaling Pathway

Images & Validation

• Tested Applications: FC, WB
• Dilution range: Flow-Cyt=1ug/Test, WB=1:500-2000
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 8H7
• Immunogen: Recombinant human TLR7 protein (24-348/1049aa)
• Target: TLR7
• Molecular Weight: 121 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

TLR-7; PRO285; TLR 7; TLR7_HUMAN; Toll like receptor 7; Toll-like receptor 7; UNQ248.

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-TLR7 antibody (orb1974655), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Recombinant human TLR7 protein, His, Primary: Anti-TLR7 (orb1974655) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kDa, Observed band size: 40 kDa.

Quick Database Links

Gene Symbol

TLR7

UniProt

Q9NYK1