TIMP3 Antibody (Center)

• Catalog Number: orb1928096
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This TIMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-139 amino acids from the Central region of human TIMP3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Equine, Rabbit, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P35625
• MW: 24145 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB21049
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Metalloproteinase inhibitor 3, Protein MIG-5, Tiss
• Note: For research use only
• NCBI: NP_000353.1

Western blot analysis of TIMP3 Antibody (Center) in HepG2 cell line lysates (35 ug/lane). TIMP3 (arrow) was detected using the purified Pab.

TIMP3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of TIMP3 Antibody (Center) Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in mouse NIH-3T3 cell line lysate. TIMP3 Antibody (Center) (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.