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Item 1 of 4
TIMP3 Antibody (Center)
Catalog Number: orb1928096
| Catalog Number | orb1928096 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB21049 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Equine, Rabbit, Rat |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 24145 Da |
| Target | This TIMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-139 amino acids from the Central region of human TIMP3. |
| UniProt ID | P35625 |
| NCBI | NP_000353.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Metalloproteinase inhibitor 3, Protein MIG-5, Tiss Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of TIMP3 Antibody (Center) in HepG2 cell line lysates (35 ug/lane). TIMP3 (arrow) was detected using the purified Pab.
TIMP3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TIMP3 Antibody (Center) Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in mouse NIH-3T3 cell line lysate. TIMP3 Antibody (Center) (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
