TIGIT Antibody / VSTM3 / VSIG9

• Catalog Number: orb607158
• Category: Antibodies
• Description: TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation). It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TIGIT/3017
• Tested applications: ELISA, FACS, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A recombinant human TIGIT protein fragment within amino acids 22-141 was used as the immunogen for the TIGIT antibody.
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This TIGIT antibody is available for research use only.
• UniProt ID: Q495A1
• Storage: Store the TIGIT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the TIGIT antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.