TH34

SKU: orb1301880

Description

TH34 is a selective inhibitor of HDAC6, HDAC8, and HDAC10 (IC50s: 4.6, 1.9, and 7.7 μM, respectively) with minimal activity against HDAC1/2/3. This compound is a valuable research tool for studying class IIb HDAC functions in oncology, neurology, and immunology research, both in vitro and in vivo.

Research Area

Epigenetics & Chromatin, Molecular Biology

Key Properties

• CAS Number: 2196203-96-8
• MW: 256.3
• Purity: 98.77% (May vary between batches)
• Formula: C₁₅H₁₆N₂O₂
• SMILES: Cc1ccc(cc1NCc1ccccc1)C(=O)NO
• Target: HDAC
• Solubility: H2O:Insoluble;DMSO:80 mg/mL (312.13 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (12.88 mM)

Bioactivity

• Target IC50:
  HDAC8:1.9 μM|HDAC10:7.7 μM|HDAC6:4.6 μM
• In Vitro:
  TH34 is well-tolerated by non-transformed human skin fibroblasts at concentrations up to 25 μM and modestly impairs colony growth in medulloblastoma cell lines, but specifically induces caspase-dependent programmed cell death in a concentration-dependent manner in several human neuroblastoma cell lines.
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• Cell Research:
  HeLa cells, stably transfected with NanoBRET plasmids NanoLuc -HDAC6 FL Fusion Vector and NanoLuc -HDAC10 FL Fusion Vector were seeded at 20,000 cells/well in white 96-well plates. Without further incubation, tracer (0.3 μM) and drugs were added in separate steps and plates were placed in a tissue culture incubator for 2 h. For NanoBRET quantification, plates were put at room temperature for 10 min. Nanoglow substrate, diluted in OptiMEM without phenol red, was added and measured within 10 min in an OPTIMA plate reader (460 nm emission for donor and 610LP filter for acceptor). The BRET signal was calculated by the ratio of acceptor signal to donor signal.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, Histone deacetylases, HDAC, HDAC10, HDAC6, HDAC8, TH 34, TH34, TH-34

Compound Identifiers

PubChem CID

134159676