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TFRC Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | WB=1:500-2000, ELISA=1:5000-10000 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Mouse CD71 protein (89-763/763aa) |
| Target | TFRC |
| Molecular Weight | 74/85 kDa |
| Purification | Affinity purified by Protein A |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD71 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Mouse TFRC Antibody, the EC50 is 21.28 ng/ml.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HT-1080 cell lysates, Primary: Anti-TFRC (orb1499425) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74/85 kDa, Observed band size: 100 kDa.
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Protocol Information
TFRC Rabbit Polyclonal Antibody (orb1499425)
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