Tenofovir hydrate

SKU: orb1909305

Description

Tenofovir hydrate is a nucleotide reverse transcriptase inhibitor used in HIV and HBV research. It exhibits antiviral activity by inhibiting viral replication, including EBV, and is employed in both in vitro and in vivo experimental studies.

Research Area

Infectious Disease & Virology, Protein Biochemistry

Key Properties

• CAS Number: 206184-49-8
• MW: 305.23
• Purity: 99.63% (May vary between batches)
• Formula: C₉H₁₆N₅O₅P
• SMILES: C([C@H](OCP(=O)(O)O)C)N1C=2C(N=C1)=C(N)N=CN2.O
• Target: HIV Protease,Reverse Transcriptase
• Solubility: DMSO:2 mg/mL (6.55 mM)

Bioactivity

• Target IC50:
  HK-2 cells:9.21 μM (48 h)
• In Vivo:
  Tenofovir Disoproxil fumarate (20, 50, 140, or 300 mg/kg) administered to BLT mice shows dose-dependent activity against vaginal HIV challenge in BLT humanized mice, significantly decreasing HIV transmission at 50, 140, and 300 mg/kg . Additionally, Tenofovir Disoproxil fumarate (0.5, 1.5, or 5.0 mg/kg/day, p.o.) causes a dose-dependent decline in serum viremia in woodchucks chronically infected with WHV, proving to be safe and effective in the woodchuck model of chronic HBV infection .
• In Vitro:
  Tenofovir hydrate exhibited cytotoxic effects in HK-2 cells, with IC50 values of 9.21 and 2.77 μM at 48 h and 72 h, respectively, in the MTT assay.Tenofovir hydrate decreased ATP levels in HK-2 cells and increased oxidative stress and protein carbonylation in the range of 3.0 to 28.8 μM. In addition, Tenofovir hydrate induced apoptosis in HK-2 cells through mitochondrial damage. The formulation of Tenofovir hydrate and M48U1 in 0.25% HEC inhibited replication of R5ophilic HIV-1BaL and X4ophilic HIV-1IIIb in activated PBMCs, as well as multiple laboratory strains and patient-derived HIV-1 strains. The combination of M48U1 and Tenofovir hydrate demonstrated synergistic antiviral activity against R5ophilic HIV-1BaL infection and was non-toxic to PBMCs.

Storage & Handling

• Storage: keep away from direct sunlight,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PMPA hydrate, GS 1278 hydrate, GS1278 hydrate

Compound Identifiers

PubChem CID

21146529