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Item 1 of 2
TCF25 Antibody (N-term)
Catalog Number: orb1934163
| Catalog Number | orb1934163 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the N-terminal region of human TCF25. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q9BQ70 |
| MW | 76667 Da |
| Tested applications | IF, WB |
| Dilution range | IF: 1:10~50, WB: 1:1000 |
| Antibody Type | Primary Antibody |
| Clone Number | RB37371 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Transcription factor 25, TCF-25, Nuclear localized Read more... |
| Note | For research use only |
| NCBI | NP_055787.1 |
TCF25 Antibody (N-term) western blot analysis in CEM cell line lysates (35 ug/lane). This demonstrates the TCF25 antibody detected the TCF25 protein (arrow).
Fluorescent image of A549 cell stained with TCF25 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). TCF25 immunoreactivity is localized to Cytoplasm significantly.
