TCF25 Antibody (N-term)

SKU: orb1934163

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB37371
• Target: This TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the N-terminal region of human TCF25.
• Molecular Weight: 76667 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Transcription factor 25, TCF-25, Nuclear localized protein 1, TCF25, KIAA1049, NULP1

TCF25 Antibody (N-term) western blot analysis in CEM cell line lysates (35 ug/lane). This demonstrates the TCF25 antibody detected the TCF25 protein (arrow).

Fluorescent image of A549 cell stained with TCF25 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). TCF25 immunoreactivity is localized to Cytoplasm significantly.

Quick Database Links

Gene Symbol

This TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the N-terminal region of human TCF25.

UniProt

Q9BQ70

RefSeq (Protein):NP_055787.1