TCEP HCl

SKU: orb1222127

Description

Tris(2-carboxyethyl)phosphine hydrochloride may be employed as a catalyst for the reduction of sulfoxides, sulfonyl chlorides, N-oxides, and azides. It may be used as a reducing agent for the reduction of various alkyl disulfides such as trans-4, 5-dihydroxy-1, 2-dithiane.

Key Properties

• CAS Number: 51805-45-9
• MW: 286.65
• Purity: >98% (HPLC)
• Formula: C₉H₁₆ClO₆P
• SMILES: Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O
• Target: Caspase
• Solubility: In Vitro: DMSO : 100 mg/mL (348.86 mM)

Bioactivity

• In Vitro:
  TCEP hydrochloride has been introduced which o ers the prospect of serving as an alternative to the more commonly employed DTT in the NF-κB-DNA binding reactions in vitro, using recombinant p50 protein and a 32P-labelled κB oligonucleotide. DTT promotes NF-κB-DNA binding in concentrations from 0.25 to 2.6 mM in binding reactions. However, in the presence of 0.25 mM DTT, inhibition of NF-κB binding is seen only at Hg2+ concentrations greater than 100 μM and results are highly variable. In contrast, TCEP hydrochloride promotes NF-κB-DNA binding in a dose-related manner in concentrations from 0.25 to 6 mM. In the presence of even 6 mM TCEP hydrochloride, Hg2+ prevents NF-κB-DNA binding at concentrations as low as 20 μM in binding reactions. The human lactoferrin (hLF) peptide is dissolved in phosphate buffer to a concentration of 0.1 mm. Reduction of the disulfide bonds is obtained by adding a 30-fold molar excess of TCEP hydrochloride with subsequent incubation for 2 h at 37 °C.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Caspase