TAT-HuR-HNS3

• Catalog Number: orb1479252
• Category: Proteins
• Description: HuR-PARP1 interaction blocker; Cell penetrating peptides.
• Modifications: None
• Structure: H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Ser-Pro-Met-Gly-Val-Asp-His-Met-Ser-Gly-Leu-Ser-Gly-Val-Asn-Val-Pro-Gly-Asn-Ala-Ser-Ser-Gly-OH
• Target Areas: Protein-protein interactions
• Form/Appearance: Freeze dried solid
• Purity: > 95% by hplc
• MW: 3696.9 Da
• Formula: C₁₅₁H₂₅₇N₅₉O₄₆S₂
• Solubility (25°C): Soluble in water
• Protein Sequence: H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Ser-Pro-Met-Gly-Val-Asp-His-Met-Ser-Gly-Leu-Ser-Gly-Val-Asn-Val-Pro-Gly-Asn-Ala-Ser-Ser-Gly-OH
• Storage: Store dry, frozen and in the dark
• Alternative names: PP370, TAT-HuR-HNS3, YGRKKRRQRRRSPMGVDHMSGLSGVNVPG
• Background: TAT-HuR-HNS3 is a cell penetrating peptide derived from the human antigen R - nucleocytoplasmic shuttling sequence (HuR-HNS) domain. HuR, also known as embryonic lethal abnormal vision-like 1 (ELAVL1) is a well characterized RNA-binding protein that increases the stability of short lived mRNAs which encode proinflammatory mediators. HuR employs its HNS domain to interact with poly(ADP-ribose) polymerase 1 (PARP1), and intervention by TAT-HuR-HNS3 interrupts this interaction, resulting in lower poly-ADP-ribosylation and decreased cytoplasmic distribution of HuR. TAT-HuR-HNS3 also blocks HuR dimerization and promotes argonaute 2-based miRNA induced silencing complex binding. TAT-HuR-HNS3 lowers the mRNA stability of proinfl ammatory mediators in TNF-α treated epithelial cells and macrophages, and decreases TNF-α induced inflammatory responses in animal lung models.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.