T4 dNMP (deoxy-Nucleotide Monophosphate) Kinase

SKU: orb85555

Description

NMP kinases catalyse the phosphorylation of nucleotide monophos-phates resulting in the corresponding nucleotide diphosphates by using ATP as a phosphate donor. T4 dNMP kinase is the only member of the family of NMP kinases that recognizes three structurally dissimilar nucleotides: dGMP, dTMP and 5-hydroxymethyl-dCMP (5-OH-dCMP).

Research Area

Signal Transduction

Images & Validation

• Application Notes:
  Activity Assay: 1 µg T4 dNMP kinase, 2 mM ATP and 2 mM dGMP in 100 mM Tris-HCl pH 7.5, 10 mM MgCl 2. Activity: One unit of enzyme catalyses the phosphorylation of 1 nmol dGMP to dGDP per minute at 37°C. Specific activity: 100 u/µg.

Key Properties

• Source: E. coli
• Biological Origin: bacteriophage T4
• Biological Activity: Specific activity: 100 u/µg.
• MW: Theoretical MW: 27 kDa
• Purity: > 90% (SDS-PAGE)

Storage & Handling

• Storage: store at -20 °C
• Form/Appearance: liquid (Supplied in 100 mM Tris-HCl pH 7.5, 10 mM MgCl 2and 50 % glycerol)
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

bacteriophage T4, recombinant, E. coli