T4 dNMP (deoxy-Nucleotide Monophosphate) Kinase

• Catalog Number: orb85555
• Category: Proteins
• Description: NMP kinases catalyse the phosphorylation of nucleotide monophos-phates resulting in the corresponding nucleotide diphosphates by using ATP as a phosphate donor. T4 dNMP kinase is the only member of the family of NMP kinases that recognizes three structurally dissimilar nucleotides: dGMP, dTMP and 5-hydroxymethyl-dCMP (5-OH-dCMP).
• Clonality: Recombinant
• Form/Appearance: liquid (Supplied in 100 mM Tris-HCl pH 7.5, 10 mM MgCl 2and 50 % glycerol)
• Purity: > 90% (SDS-PAGE)
• MW: Theoretical MW: 27 kDa
• Application notes: Activity Assay: 1 µg T4 dNMP kinase, 2 mM ATP and 2 mM dGMP in 100 mM Tris-HCl pH 7.5, 10 mM MgCl 2. Activity: One unit of enzyme catalyses the phosphorylation of 1 nmol dGMP to dGDP per minute at 37°C. Specific activity: 100 u/µg.
• Source: E. coli
• Biological Origin: bacteriophage T4
• Biological Activity: Specific activity: 100 u/µg.
• Storage: store at -20 °C
• Note: For research use only