Swine IgM Antibody

SKU: orb346399

Description

Swine IgM Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Swine IgM whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Key Properties

• Source: Swine
• Biological Origin: Swine
• Isotype: IgM
• Purity: Swine IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Swine IgM whole molecule was assayed by immunoelectrophoresis resulting in a single precipitin arc against anti-Swine Serum and anti-Swine IgM (µ chain specific). No reaction was observed against anti-Swine IgG F(c). Some light chain cross reactivity will occur with anti-Swine IgG.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. Swine IgM whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.1% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Swine immunoglobulin M, Pig IgM

SDS-PAGE of Swine IgM Whole Molecule. Lane 1: Swine IgM, 1.0 µg, Non-reduced. Lane 2: Swine IgM, 5.0 µg, Non-reduced. Lane 3: Swine IgM, 10.0 µg, Non-reduced. M: Opal Pre-stained Marker. Lane 4: Swine IgM, 1.0 µg, Reduced. Lane 5: Swine IgM, 5.0 µg, Reduced. Lane 6: Swine IgM, 10.0 µg, Reduced. Predicted/Observed size - Non-Reduced: 900 kDa, Reduced - 75, 25 kDa.