Swine IgG Fc Antibody Peroxidase Conjugated

• Catalog Number: orb1463549
• Category: Antibodies
• Description: Swine IgG F(c) antibody (HRP)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Porcine
• Isotype: IgG
• Immunogen: Swine IgG F(c) fragment
• Antibody Type: Secondary Antibody
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:171,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgG, Swine IgG F(c) and Swine Serum. No reaction was observed against Swine IgG F(ab) .
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Rabbit Anti-Swine IgG F(c) peroxidase Conjugated A
• Note: For research use only
• Application notes: Anti-Swine IgG F(c) Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Rabbit Anti-Swine IgG F(c) Antibody Peroxidase Conjugated tested against purified Swine IgG F(c) HRP. Each well was coated in duplicate with 1.0 µg of Swine IgG F(c) (p/n orb2652737) [Red Line] and Swine IgG (p/n orb2652739) [Green Line]. The working dilution of Swine IgG F(c) is 1:219000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer and TMB substrate (p/n orb348651).