SMT3 Antibody

SKU: orb344612

Description

SUMO antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Application Notes:
  Rabbit Anti-Sumo antibody reacts yeast SUMO tested by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1,000 dilution is recommended. A 12 kDa band corresponding to yeast SUMO is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. For ELISA a 1:1,000 to 1:5,000 dilution is recommended. Researchers should determine optimal titers for other applications.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast SUMO protein.
• Target: SMT3
• Purity: Anti-Sumo Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-SUMO Antibody, Ubiquitin-like protein SMT3 antibody, SMT3 antibody, Ubiquitin like protein of the SUMO family antibody, SMT3_YEAST antibody, DmSUMO 1 antibody

Western blot of SUMO-GFP fusion proteins cleaved by insect cell protein extracts. Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 µg E. coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 µg total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with Anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60°C and were then re-probed using the Anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4°C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey Anti-Rabbit IgG (orb347758) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.

Quick Database Links

Gene Symbol

SMT3

UniProt

Q12306

RefSeq:6320718