Sucrose Phosphorylase Antibody

SKU: orb344205

Description

Sucrose phosphorylase antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: E. coli
• Application Notes:
  Anti-Sucrose phosphorylase Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Sucrose Phosphorylase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,500 to 1:32,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Sucrose Phosphorylase [E.coli]
• Purity: Anti-Sucrose phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.coli]. Cross reactivity against Sucrose Phosphorylase from other sources is unknown.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

goat anti-Sucrose Phosphorylase Antibody, Putative sucrose phosphorylase, Sucrose glucosyltransferase

Biorbyt Goat anti Sucrose Phosphorylase antibody was used to detect purified Sucrose Phosphorylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/orb348637 1 hr RT).

Western Blot of Goat Anti-Sucrose Phosphorylase Antibody. Lane 1: Opal Prestained Ladder. Lane 2: HeLa Lysate (10 µg) spiked with Sucrose Phosphorylase protein (50 ng). Lane 3: HeLa Lysate (10 µg) spiked with Sucrose Phosphorylase protein (20 ng). Lane 4: HeLa Lysate (10 µg) spiked with Sucrose Phosphorylase protein (10 ng). Lane 5: HeLa Lysate (10 µg) (p/n orb348668). Primary Antibody: Anti-Sucrose Phosphorylase at 1 µg/ml overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG HRP mx8 custom at 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644). Expect/Observe MW: ~50kDa.

Quick Database Links

UniProt

P76041

RefSeq:YP_852475.1