Streptavidin Fluorescein Conjugated

• Catalog Number: orb348766
• Category: Tools
• Description: Streptavidin Fluorescein Conjugated
• Tested applications: DOT, FC, FLISA, IF
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from chromatographically pure Streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Streptavidin.
• Conjugation: FITC
• Hazard Information: Non-Toxic
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: SA, S avidin, streptococcus avidin, streptavidin F
• Note: For research use only
• Application notes: Streptavidin Fluorescein Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot of STREPTAVIDIN Fluorescein Conjugated. Lane A: BSA-Biotin. Lane B: BSA. Lanes 1: load 100 ng, 2-5: serial dilution 3 fold. Primary Antibody: n/a Secondary Antibody: Streptavidin Fluorescein Conjugated at 1 mg/mL at RT for 30 minutes. Block: orb348637 at RT for 30 minutes.