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Catalog Number | orb1238868 |
---|---|
Category | Antibodies |
Description | StrepII-tag Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Tag |
Isotype | IgG |
Immunogen | Anti-StrepII-tag antibody (orb1238868) StrepII-tag antibody was raised against the amino acid sequence "asWSHPQFEKga". |
Concentration | 1 mg/mL |
Dilution range | WB: 0.25-1 μg/mLAntibody validated: StrepII-tag antibody can be used for the detection of StrepII-tagged proteins |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | StrepII-tag antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | StrepII-tag Antibody: Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot Validation with StrepII-tagged Recombinant Protein. Loading: A 5 ng and B 25 ng of StrepII-tagged recombinant protein. Antibodies: StrepII-tag orb1238868 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with StrepII-tagged Recombinant Protein. Loading: 30 ng of StrepII-tagged recombinant protein per lane. Antibodies: StrepII-tag orb1238868, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 µg/mL, Lane 2: 0.5 µg/mL, Lane 3: 1 µg/mL.
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