SPRN Antibody (C-term)

• Catalog Number: orb1930834
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB25483
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 14522 Da
• Target: This SPRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-105 amino acids from the C-terminal region of human SPRN.
• UniProt ID: Q5BIV9
• NCBI: NP_001012526.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Shadow of prion protein, Protein shadoo, SPRN, SHO
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SPRN Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPRN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of lysate from human brain tissue lysate, using SPRN Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.