SPHK1 Antibody (Center)

SKU: orb1929453

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:2000, IHC-P: 1:25, IHC-P: 1:25
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB03621
• Target: This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
• Molecular Weight: 42518 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK

Staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Anti-SPHK1 Antibody (Center) at 1:2000 dilution + human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

All lanes: Anti-SPHK1 Antibody (Center) at 1:2000 dilution. Lane 1: human liver lysate. Lane 2: HUVEC whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.

UniProt

Q9NYA1

RefSeq (Protein):NP_068807.2, NP_892010.2, NP_001136074.1, NP_001136073.1