SPAG16 Antibody

• Catalog Number: orb1256360
• Category: Antibodies
• Description: SPAG16 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human SPAG16 (NP_001020607.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 71kDa
• Target: SPAG16
• UniProt ID: Q8N0X2
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SPAG16, SPAG16, WDR29, WD repeat domain 29, sperm-
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse testis, using SPAG16 antibody (orb1256360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human brain cancer using SPAG16 antibody (orb1256360) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using SPAG16 antibody (orb1256360) at dilution of 1:100 (40x lens).