SPAG16 Antibody

SKU: orb1939902

Description

Anti-SPAG16 Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution range: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml, -
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E.coli-derived human SPAG16 recombinant protein (Position: D28-F619). Human SPAG16 shares 75.1% amino acid (aa) sequence identity with mouse SPAG16.
• Target: Sperm-associated antigen 16 protein
• Molecular Weight: 71 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

PF20; Pf20 protein homolog; SPAG16; sperm associated antigen 16; WDR29

Flow Cytometry analysis of SH-SY5Y cells using anti-SPAG16 antibody. Overlay histogram showing SH-SY5Y cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPAG16 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Western blot analysis of SPAG16 using anti-SPAG16 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPAG16 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SPAG16 at approximately 71 kDa. The expected band size for SPAG16 is at 71 kDa.

Quick Database Links

Gene Symbol

Sperm-associated antigen 16 protein

UniProt

Q8N0X2